学术论文

      去甲基蟛蜞菊内酯对香烟烟雾干预NHBE细胞损伤的保护作用

      Protective Effect of Demethylwedelolactone on Cigarette Smoke Extract-Induced Cytotoxicity of NHBE Cells

      摘要:
      考察去甲基蟛蜞菊内酯(Demethylwedelolactone, DWEL)对肺支气管上皮细胞(Normal Human Bronchial Epithelial,NHBE)的保护作用.以香烟烟雾提取物(Cigarette Smoke Extract,CSE)干预NHBE细胞为模型,评价DWEL对模型细胞存活率的影响.进一步采用超氧化物歧化酶(Superoxide Dismutase,SOD)和 丙二醛(Malondialdehyde,MDA)试剂盒评价DWEL对细胞氧化损伤的影响;采用Western blot对炎症指标环氧化酶-2 (Cyclooxygenase, COX-2)和细胞间黏附分子-1(Intercellular Cell Adhesion Molecule-1, ICAM-1)进行测定.结果显示,DWEL能显著提高CSE干预的NHBE细胞的存活率(70.1%),提高SOD的活力,下调MDA的含量, 使COX-2和ICMA-1的表达显著降低,并向正常方向接近.表明DWEL能降低CSE干预的NHBE损伤.
      Abstract:
      This study is to investigate the protection effect of demethylwedelolactone on NHBE cells.The CSE induced NHBE cells model was built up to evacuate the cell viability.SOD and MDA kit were used to evaluate the effect of DWEL on cell oxidative damage and COX-2 and ICAM-1 were measured by Western blot.The results showed that DWEL could significantly increase the survival rate of NHBE cells induced by CSE (70.1%).Pretreatment with DWEL significantly reversed the activity of SOD and the level of MDA to normal level.The protein expression levels of COX-2 and ICAM-1which related to inflammatory response were remarkablely reduced by DWEL compared with 10%CSE treatment.These results suggested that DWEL can reduce CSE-induced injury on NHBE cells
      Author: DING Shumin SONG Guoqiang ZHANG Ye LI Jianpeng CHEN Xiangxiang XU Defeng
      作者单位: 常州大学 制药与生命科学学院,江苏 常州,213164
      年,卷(期): 2017, 29(4)
      分类号: R285
      在线出版日期: 2017年8月14日
      基金项目: 国家自然科学基金资助项目,江苏省产学研前瞻项目