学术论文

      食品中常见有机磷农药残留的气相色谱质谱检测方法

      Detection of organophosphorus pesticide residues in food by gas chromatography-mass spectrometry

      摘要:
      建立了气相色谱质谱联用技术分析蔬菜、谷物中敌敌畏、甲胺磷等16种有机磷农药残留的快速检测方法。样品用乙腈提取,采用QuEChERS处理方法进行前处理,用气相色谱质谱联用多反应监测仪检测16种有机磷农药残留。16种有机磷农药在15 min内流出,分离度良好,农药在0?025~0?4 mg/kg范围内线性良好,相关系数( R2)均大于0?94。不同基质如大米、面粉、蔬菜的加标回收率分别为75?9%~112?2%、77?3%~126?9%、76?2%~125?3%,相对标准偏差( RSD)<8?5%。重复性实验引入的不确定度符合国家标准。该方法具有操作方便、分离时间短、灵敏度高、选择性好等优点,适用于大多数食品中有机磷农药残留检测。
      Abstract:
      To establish a rapid detection method for the determination of dichlorvos,methamidophos and other organophosphorus pesticide residues in vegetables and grains by gas chromatography?mass spectrometry ( GC?MS ) , samples were first extracted with acetonitrile and pretreated by QuEChERS method,then detected by gas chromatography?mass spectrometry under multiple reaction monitoring. 16 kinds of organophosphorus pesticides could be detected within 15 min with a good separating degree and good linearity. The correlation coefficient range was 0?025?0?4 mg/kg, and the correlation coefficient (R2) were more than 0?94.The standard addition recovery rates of different substrates such as rice,flour, and vegetable were in the ranges of 75?9%?112?2%, 77?3%?126?9%, and 76?2%?125?3% with relative standard deviation ( RSD )<8?5%. The uncertainty of the repeated experiments met the national standards. The method was simple, rapid, high sensitivity, good selectivity, and was suitable for determination of organophosphorus pesticides residues in food.
      Author: CUI Ruyu LI Yi XIONG Xiaohui
      作者单位: 南京工业大学 食品与轻工学院,江苏 南京,211800
      年,卷(期): 2017, 39(1)
      分类号: O657.63
      在线出版日期: 2017年3月7日
      基金项目: 国家科技支撑计划(2012BAK17B09);江苏省科技基础设施建设计划