学术论文

      基于大肠杆菌MG1655载体矿化合成纳米硒的研究

      Biomineral synthesis of nano-selenium with Escherichia coli MG1655 as intermediary

      摘要:
      根据生物矿化策略,以不同质量浓度的Na2SeO3溶液作为硒源,利用大肠杆菌MG1655生物还原Na2SeO3制备纳米硒.研究Na2SeO3质量浓度对大肠杆菌生长曲线的影响,用氢化物原子荧光光谱法(HG-AFS)测定Na2SeO3的还原效率,进一步利用扫描电子显微镜(SEM)、X线衍射仪(XRD)和傅里叶红外光谱(FT-IR)等仪器对产物进行了相关表征.结果表明:大肠杆菌MG1655能将Na2SeO3大量矿化为粒径基本在100~250 nm左右的红色单质纳米硒颗粒,且对各质量浓度下的Na2SeO3的还原率均在50%以上.
      Abstract:
      The reduction with Escherichia coli MG1655 of sodium selenite was used to prepare nano-selenium with different mass concentrations of sodium selenite as selenium source artificially.The influence of sodium selenite mass concentration on the growth curve of Escherichia coli MG1655 was investigated.The reduction efficiency of sodium selenite was determined by hydride generation atomic fluorescence spectrometry (HG-AFS),and the structure of the product was characterized by scanning electron microscope (SEM),X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).Results indicated that sodium selenite could be biomineralized in abundance by Escherichia coli MG1655 to nano-selenium particles in red simple substance with their diameters mainly 100~250 nm.Moreover,all the reduction rates of various mass concentrations of sodium selenite solution were over 50%.
      作者: 武童 [1] 汪振炯 [2] 华春 [2] 周峰 [3] 王仁雷 [4] 黄和 [3]
      Author: WU Tong [1] WANG Zhenjiong [2] HUA Chun [2] ZHOU Feng [3] WANG Renlei [4] HUANG He [3]
      作者单位: 南京工业大学 生物与制药工程学院,江苏南京211800;南京晓庄学院 食品科学学院,江苏南京211171 南京晓庄学院 食品科学学院,江苏南京,211171 南京工业大学 生物与制药工程学院,江苏南京,211800 江苏第二师范学院 生物系,江苏南京,210013
      年,卷(期): 2017, 39(3)
      分类号: Q936
      在线出版日期: 2017年7月3日
      基金项目: 国家自然科学基金,江苏省自然科学基金