学术论文

      活血定眩胶囊含药血清减轻小鼠脑微血管内皮细胞bEnd.3缺氧损伤

      Protective effect of Huoxue-Dingxuan capsule-medicated serum on mouse brain microvascular endothelial cell line bEnd.3 with hypoxic injury

      摘要:
      目的:研究活血定眩胶囊含药血清对抗缺氧所致小鼠脑微血管内皮细胞bEnd.3损伤的作用.方法:将30只大鼠随机分为空白对照组15只和活血定眩胶囊组15只,采集2组大鼠血清.将bEnd.3细胞分为正常组、缺氧模型组和活血定眩胶囊血清组,给药后,bEnd.3细胞缺氧6 h.显微镜下观察细胞形态,流式细胞术检测细胞凋亡率及细胞周期,按试剂盒方法检测细胞上清液超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量.结果:活血定眩胶囊含药血清可显著对抗缺氧造成的损伤,明显改善bEnd.3细胞的形态,使缺氧所致细胞凋亡数明显减少,有效抑制缺氧诱导的bEnd.3细胞发生G1/S期阻滞,抑制MDA生成,增强SOD活性.结论:活血定眩胶囊对缺氧所致bEnd.3细胞的损伤具有明显的对抗作用,其机制与增强细胞抗氧化能力、抑制细胞凋亡有关.
      Abstract:
      AIM:To investigate the protective effect of Huoxue-Dingxuan capsule-medicated serum on mouse brain microvascular endothelial cell line bEnd .3 with hypoxic injury .METHODS:The Wistar rats were randomly divided into control group and Huoxue-Dingxuan capsule group .The serum was collected at the 7th day after drug administration . The bEnd.3 cells were divided into normal group , hypoxia serum model group and Huoxue-Dingxuan capsule serum group . After administration of the medicated serum , bEnd.3 cell were treated with hypoxia for 6 h.The cell morphology was ob-served under microscope , the apoptosis rate and cell cycle were detected by flow cytometry , and the activity of superoxide dismutase ( SOD) and the content of malondialdehyde ( MDA) were detected by ELISA .RESULTS:Compared with other groups, Huoxue-Dingxuan capsule-medicated serum significantly resisted the injury caused by hypoxia , obviously improved the morphology of bEnd .3 cells, significantly reduced the number of apoptotic cells induced by hypoxia , and effectively in-hibited the occurrence of hypoxia-induced G1/S phase arrest in the bEnd.3 cells.At the same time, the medicated serum inhibited MDA production , and increased SOD activity .CONCLUSION: Huoxue-Dingxuan capsule attenuates hypoxia-induced bEnd .3 cell damage by enhancing the antioxidant capacity of cells and inhibiting cell apoptosis .
      作者: 刘涛 [1] 宋敏 [1] 巩彦龙 [1] 周灵通 [1] 董万涛 [2] 刘建鸿 [1]
      Author: LIU Tao [1] SONG Min [1] GONG Yan-long [1] ZHOU Ling-tong [1] DONG Wan-tao [2] LIU Jian-hong [1]
      作者单位: 甘肃中医药大学,甘肃兰州,730000 甘肃中医药大学附属医院,甘肃兰州,730020
      刊 名: 中国病理生理杂志 ISTICPKU
      年,卷(期): 2017, 33(3)
      分类号: R363
      在线出版日期: 2017年4月10日
      基金项目: 国家自然科学基金资助项目,甘肃省自然科学基金资助项目,甘肃省财政厅基本科研业务课题,甘肃省中药现代制造工程研究院项目