学术论文

      14-3-3蛋白在宫颈癌组织中的表达及其意义

      Expression of 14-3 -3 protein in the cervical cancer patients and its clinical significance

      摘要:
      目的:探讨14-3-3蛋白在宫颈癌发病机制中的作用。方法选取宫颈癌患者107例,取癌组织(宫颈癌组),并与50例宫颈上皮内瘤变(宫颈上皮内瘤变组)、慢性宫颈炎(慢性宫颈炎组)作对照,免疫组化法检测14-3-3蛋白的表达。结果宫颈癌组14-3-3蛋白表达的阳性率(75.70%)显著高于宫颈上皮内瘤变组和慢性宫颈炎组(分别为42.00%、30.00%)(χ2=17.00、29.96,均 P <0.01),宫颈上皮内瘤变组与慢性宫颈炎组14-3-3蛋白表达的阳性率差异无统计学意义(χ2=1.56,P >0.05)。在宫颈癌组中,伴有淋巴结转移组14-3-3蛋白的阳性率(88.89%)显著高于不伴有淋巴结转移组(63.16%)(χ2=5.15,P <0.05)。结论14-3-3蛋白在宫颈癌组织中表达增强,可能与宫颈癌的恶性分化及转移相关,14-3-3蛋白可能成为一种治疗肿瘤的新靶点。
      Abstract:
      Objective To investigate the potential roles of 14 -3 -3 in the pathogenesis of the cervical cancer.Methods Tissues from 107 patients with cervical cancer(cervical cancer group),50 cases of cervical intra-epithelial neoplasia (cervical intraepithelial neoplasia group)and 50 cases of chronic cervicitis (chronic cervicitis group)were obtained.Immunohistochemistry was performed to determine the expression of 14 -3 -3 protein in tissues of the three groups.Results The positive rate of 14 -3 -3 in cervical cancer group(75.70%)was signifi-cantly higher than that in cervical intraepithelial neoplasia group and chronic cervicitis group(42.00%,30.00%, respectively)(χ2 =17.00,29.96,all P <0.01),while there was no significant difference of 14 -3 -3 level between the cervical intraepithelial neoplasia group and chronic cervicitis group(χ2 =1.56,P >0.05).Moreover,in the cervi-cal cancer group,the positive rate of 14 -3 -3 in lymph node metastasis group was 88.89%,which was higher than that in negative lymph node metastasis group (63.16%)(χ2 =5.15,P <0.05).Conclusion The expression level of 14 -3 -3 protein in cervical cancer is increased,it may be correlated with cervical cancer cell malignant differenti-ation and metastasis.Perhaps,14 -3 -3 will play a key role as a novel target for tumor therapy.
      作者: 赵巧云 [1] 范广民 [2] 阮正英 [3] 应香岚 [4]
      Author: Zhao Qiaoyun [1] Fan Guangmin [2] Ruan Zhengying [3] Ying Xianglan [4]
      作者单位: 台州市中西医结合医院妇产科, 浙江省温岭,317523 台州市中心医院病理科, 浙江省台州,317000 台州市中西医结合医院病理科, 浙江省温岭,317523 台州市肿瘤医院病理科, 浙江省温岭,317500
      刊 名: 中国基层医药 ISTIC
      年,卷(期): 2017, 24(6)
      在线出版日期: 2017年3月29日
      基金项目: 浙江省温岭市科技计划项目(温科[2012]12号)Fund programScience and technology planning project of Wenling city,Zhejiang province