学术论文

      吡格列酮对腹腔巨噬细胞源泡沫细胞形成及 TLR4表达的影响

      Effects of pioglitazone on expressions of TLR4 in peritoneal macrophage foam cell

      摘要:
      目的:观察吡格列酮对腹腔巨噬细胞源泡沫细胞的形成及 TLR4表达的影响。方法体外培养泡沫细胞分四组即正常对照组、吡格列酮组、氧化型低密度脂蛋白(ox-LDL)组和 ox-LDL +吡格列酮组。ox-LDL(50 mg/mL)、吡格列酮(20 mmol/L)作用于腹腔巨噬细胞24 h,油红 O 染色观察各组泡沫细胞的形成。采用免疫组化检测观察各组 TLR4的表达。结果对照组及吡格列酮组细胞几乎不见油红 O 染色,ox-LDL组大量细胞胞质被油红 O 染成暗红色,而 ox-LDL +吡格列酮组细胞胞质染色明显浅于 ox-LDL 组细胞。ox-LDL 组泡沫细胞 TLR4的表达明显高于对照组和吡格列酮组(24 h:t =3.015、t =4.007;48 h:t =3.100,t =4.008,均 P <0.01)。而ox-LDL +吡格列酮组泡沫细胞 TLR4的表达明显低于 ox-LDL 组(24 h:t =4.075;48 h:t =4.150,均 P <0.05)。结论吡格列酮可能通过抑制 ox-LDL 介导的巨噬细胞 TLR4的表达,抑制泡沫细胞的形成,而起到抗 AS 的作用。
      Abstract:
      Objective To study the effects of Pioglitazone on TLR4 expression and peritoneal macrophage foam cells formation induced by ox - LDL.Methods Foam cells were cultured equally divied into four groups in random:the normal control,pioglizaone group,ox-LDL group and ox-LDL +pioglizaone group.The SD rats peritoneal macrophages were pretreated with ox - LDL(50 mg/mL)and Pioglitazone(20 mmo1 /L)respectively for 24 h.Then they were stained by oil red O to determine foam cells formation.The effects of Pioglitazone on foam cells TLR4 ex-pressions were detected by immunohistochemistry.Results The SD rats peritoneal macrophages of control group and Pioglitazone group are not almost stained by oil red O,but the macrophages of ox-LDL group become dark red after stained by oil red O,and the cells color of ox-LDL + Pioglitazone group is thinner than that of ox-LDL group.The expressions of TLR4 in ox-LDL group are higher than those in control and Pioglitazone group significantly(24 h,t =3.015,t =4.007;48 h,t =3.100,t =4.008,P <0.01).while expressions in ox-LDL +Pioglitazone group are lower than ox-LDL group respectively(24 h,t =4.075;48 h,t =4.150,P <0.05).Conclusion Pioglitazone might inhibit the formation of foam cells by suppressing the expressions of TLR4 in macrophages.
      作者: 蒋伟 王书梅
      Author: Jiang Wei Wang Shumei
      作者单位: 湖南中医药大学附属衡阳医院心内科, 湖南省衡阳,421001
      刊 名: 中国基层医药 ISTIC
      年,卷(期): 2017, 24(6)
      在线出版日期: 2017年3月29日
      基金项目: 广东省韶关市科技计划项目(2012CX/K107);广东省韶关市卫生局科研课题(Y14028)Fund programFund programScience and Technology planning project of shaoguan city,Guangdong province (2012CK/K107);Health bureau of shaoguan city,Guangdong province