学术论文

      快律宁胶囊及其含药血清对大鼠心室肌细胞钙离子浓度的影响

      Influence of Kuailvning capsule and its drug serum on the concentration of Ca2 + in rat ventricular muscle cells

      摘要:
      目的:研究快律宁胶囊(KLN)含药血清对大鼠心室肌细胞钙离子(Ca2+)浓度的影响。方法将 SD 大鼠采用数字表法随机分为空白对照组、血清对照组、KLN 高、中、低剂量组,每组各6只,用酶解法急性分离大鼠心室肌细胞,采用 Fluo-3/AM荧光探针测定不同浓度的 KLN 及其含药血清对单个大鼠心室肌细胞内 Ca2+浓度的影响,比较不同组别 Ca2+浓度。结果与对照组比较,KLN 高、中、低剂量组在50 s、100 s、150 s、200 s、250 s 后胞浆 Ca2+平均荧光强度分别为[(18.75±1.55)、(16.69±0.93)、(17.76±1.26)],[(25.47±1.118)、(17.86±1.49)、(17.81±1.13)],[(29.05±1.31)、(20.14±1.73)、(18.26±1.37)],[(35.21±1.33)、(23.19±0.97)、(18.18±1.46)],[(41.08±1.21)、(26.34±1.69)、(17.91±1.01)],均明显高于对照组(F =5.556、7.007、8.816、10.208、12.232,均 P <0.01)。与对照组相比,KLN 高剂量含药血清组50 s、100 s、150 s、200 s、250 s 后胞浆 Ca2+平均荧光强度分别为(17.85±1.69)、(18.95±1.73)、(21.85±1.39)、(23.01±1.48)、(24.07±1.42),明显高于血清对照组(t =3.642、4.406、6.074、7.402、8.625,P <0.01),中剂量含药血清组100 s、150 s、200 s、250 s 后胞浆 Ca2+平均荧光强度分别为(18.01±1.42)、(18.76±1.22)、(19.73±1.37)、(21.04±1.16),明显高于血清对照组(t =3.902、4.033、4.126、4.326,均 P <0.01),而 KLN 低剂量含药血清组对 Ca2+平均荧光强度的影响差异无统计学意义(P >0.05)。结论 KLN 含药血清能促进心肌细胞自发的外钙内流,说明 KLN 含药血清具有抗心律作用且其与对 Ca2+通道的作用有关。
      Abstract:
      Objective To observe the influence of Kuailvning capsule(KLN)and its drug serum on the concentration of Ca2 + in rat ventricular muscle cells.Methods SD rats were randomly divided into five groups:the control group,serum control group,KLN high -dose group,KLN medium -dose group and KLN low -dose group,six mice in each group.The rat ventricular myocytes were separated by enzymatic hydrolysis.The influence of KLN and its drug serum on the concentration of Ca2 + in rat ventricular muscle cells were observed by Fluo -3 /AMprobe.Results The mean fluorescence intensity of cytosolic Ca2 + in the KLN high -dose group,KLN medium -dose group and KLN low -dose group after 50s,100s,150s,200s,250s were [(18.75 ±1.55),(16.69 ±0.93),(17.76 ±1.26)], [(25.47 ±1.118),(17.86 ±1.49),(17.81 ±1.13)],[(29.05 ±1.31),(20.14 ±1.73),(18.26 ±1.37)], [(35.21 ±1.33),(23.19 ±0.97),(18.18 ±1.46)],[(41.08 ±1.21),(26.34 ±1.69),(17.91 ±1.01)], which were higher than those in the control group(F =5.556,7.007,8.816,10.208,12.232,all P <0.01 ).The mean fluorescence intensity of cytosolic Ca2 + in the KLN high -dose group after 50s,100s,150s,200s,250s were (17.85 ±1.69),(18.95 ±1.73),(21.85 ±1.39),(23.01 ±1.48),(24.07 ±1.42),which were higher than those in the serum control group(t =3.642,4.406,6.074,7.402,8.625,all P <0.01 ).The mean fluorescence intensity of cytosolic Ca2 + in the KLN medium -dose group after 100s,150s,200s,250s were (18.01 ±1.42), (18.76 ±1.22),(19.73 ±1.37),(21.04 ±1.16),which were higher than those in the serum control group(t =3.902,4.033,4.126,4.326,all P <0.01).There were no statistically significant difference between the KLN low -dose group and the serum control group(P >0.05).Conclusion KLN drug serum can promote myocardial cell spon-taneous extracellular calcium influx,the mechanism of KLN antiarrhythmic effect may be related to the Ca2 + channel.
      作者: 胡华杰 [1] 郭燕娜 [1] 温慧萍 [2]
      Author: Hu Huajie [1] Guo Yanna [1] Wen Huiping [2]
      作者单位: 舟山市中医院药剂科, 浙江省舟山,316000 丽水学院, 浙江省丽水,323000
      刊 名: 中国基层医药 ISTIC
      年,卷(期): 2017, 24(6)
      在线出版日期: 2017年3月29日