学术论文

      紫砂芸豆花色苷的超声/微波协同提取研究及其组成分析

      Research on Ultrasonic/Microwave Synergistic Extraction of Anthocyanins from Purple Kidney Bean and Structure Identification

      摘要:
      以东北紫砂芸豆为原料,采用单因素试验和响应面优化试验研究超声/微波协同提取紫砂芸豆中花色苷的工艺参数,并采用液质连用色谱法对提取物进行不同浓度乙醇洗脱,进行组分分析.结果表明,超声/微波协同提取紫砂芸豆花色苷的有效工艺参数为:微波功率251 W,液料比13.7 mL/g,提取时间24.9min.不同浓度乙醇洗脱液的色谱分析发现,提取物中主要有5种花色苷物质,其中天竺葵素-3-葡萄糖苷、芍药素-3-葡萄糖苷含量较高,且主要集中在40%乙醇洗脱液中.本研究为大孔树脂纯化紫砂芸豆花色苷提供了参考,为紫砂芸豆花色苷的提取加工及工业应用提供依据.
      Abstract:
      In this paper,purple kidney bean varieties from northeast of China was taken as the raw material,the ultrasonic/microwave synergistic extraction process of anthocyanins from purple bean were studied,and the structure was identified.As indicated in the results,the optimum ultrasonic/microwave synergistic extraction process parameters of anthocyanins from purple kidney bean were shown as following:microwave power 251 W,liquid to solid ratio 13.7 mL/g,extraction time 24.9 min.Chromatographic analysis of ethanol eluent with different concentration showed that there were mainly five kinds of anthocyanins substances,in which pelargonidin 3-glucoside,peony-3-glucoside were higher by of different concentration ethanol elution liquid quality analysis,pelargonidin3-glucoside,peony prime3-glucoside mainly concentrated in 40% ethanol eluent.The research may provide reference for anthocyanin purification with macroporous resin,and as theory base for anthocyanin process and its industrial application.
      作者: 李良玉 [1] 宋大巍 [2] 刁静静 [3] 贾鹏禹 [3] 张丽萍 [4]
      Author: Li Liangyu [1] Song Dawei [2] Diao Jingjing [3] Jia Pengyu [3] Zhang Liping [4]
      作者单位: 黑龙江八一农垦大学国家杂粮工程技术研究中心,大庆163319;东北林业大学林学院,哈尔滨 150040 黑龙江八一农垦大学食品学院,大庆,163319 黑龙江八一农垦大学国家杂粮工程技术研究中心,大庆,163319 黑龙江八一农垦大学国家杂粮工程技术研究中心,大庆163319;黑龙江八一农垦大学食品学院,大庆163319
      刊 名: 中国粮油学报 ISTICPKU
      年,卷(期): 2017, 32(3)
      分类号: TS214.9
      在线出版日期: 2017年4月18日
      基金项目: 国家科技支撑计划