首頁 > 期刊首頁 > 國際眼科雜志 > 2007年1期 > 經羥基磷灰石處理的純鈦表面有利于角膜成纖維細胞的粘附和增殖
經羥基磷灰石處理的純鈦表面有利于角膜成纖維細胞的粘附和增殖
Hydroxyapatite modified titanium promotes superior adhension and proliferation of corneal fibroblast in comparison with pure titanium
- doi：
- 摘要：
- 目的:研究與純鈦相比,經羥基磷灰石表面改性處理的純鈦是否能增加兔角膜基質成纖維細胞在其表面的粘附和增殖.方法:利用生物活性的羥基磷灰石對純鈦進行表面改性.將第4代兔角膜基質成纖維細胞接種于羥基磷灰石表面改性的鈦、純鈦及玻璃表面,24,48,72 h后,使用丫啶橙染色的方法觀察材料表面細胞的粘附和增殖情況,電鏡觀察材料表面的細胞形態.結果:各個時間點,羥基磷灰石表面改性的鈦表面的細胞數都多于其他材料(P＜0.05).24 h,羥基磷灰石表面改性的鈦表面和玻璃表面的細胞擴展面積要大于純鈦表面.72 h電鏡觀察發現羥基磷灰石表面改性的鈦表面的細胞擴展面積最大,細胞張力絲最長.結論:與純鈦相比,經羥基磷灰石表面改性處理的純鈦可增加兔角膜基質成纖維細胞在其表面粘附和增殖
- Abstract：
- AIM: To determine whether hydroxyapatite modified titanium promotes superior adhension and proliferation of rabbit corneal fibroblast in comparison with pure titanium.METHODS: We used bioactive hydroxyapatite to modify titanium surfaces. Fourth passage fibroblasts of rabbit cornea were seeded on hydroxyapatite modified titanium surfaces, pure titanium and glass surfaces. Cell adhension, proliferation and morphology were detected at 24 hours, 48 hours, and 72hours using a acridine orange stain. Further studies of cell morphology were performed using scanning electron microscopy.RESULT: Ceil counts were significantly greater on hydroxyapatite modified titanium surfaces at each time point(P＜0.05).At 24 hours, cell spreading was greater on hydroxyapatite-coated titanium and glass than on the pure titanium. At 72 hours, compared with pure titanium and glass surfaces, the cells on hydroxyapatite modified titanium surfaces had greater spreading area and longer stress fibers.CONCLUSIONS: Hydroxyapatite modified titanium promotes superior adhension and proliferation of rabbit corneal fibroblast in comparison with pure titanium.
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