高级检索    帮助
  • 贝克曼库尔特商贸（中国）有限公司
  • 第二届中国流感论坛
  • 万方数据诚聘英才
  • 数字图书馆论坛
  • 万方数据中华医学会


相关检索词

dna提取 elisa enos mda nitrate no2 nos pathogenesis pe rflp rt rt-pcr so2 sod 发病机制 弓形虫 基因克隆 沙门氏菌 系统发育 原位杂交

浏览历史

首页 > 学术期刊 > 华中科技大学学报（医学）（英德文版） > 2009年2期 > Endothelial Nitric Oxide Synthase Traffic Inducer in the Umbilical Vessels of the Patients with Pre-eclampsia

Endothelial Nitric Oxide Synthase Traffic Inducer in the Umbilical Vessels of the Patients with Pre-eclampsia

The expression of endothelial nitric oxide synthase traffic inducer (NOSTRIN) was examined in the umbilical vessels of the patients with pre-eclampsia (PE) to explore its possible role in the pathogenesis of PE.The NOSTRIN mRNA in umbilical tissues was determined by RT-PCR.The eNOS activity in umbilical vessels was spectrophotometrically detected.NO2-/NO3-,the stable metabolic end products of NO,was measured by using nitrate reductase.RT-PCR showed that the expression level of NOSTRIN was significantly higher in women with PE than in the normal group (P<0.01).The activity of eNOS was significantly decreased in PE group [(12.83±3.61) U/mg] than in normal group [(21.72±3.83) U/mg] (P<0.01).The level of NO2-/NO3- in PE patients (27.53± 7.48) μmol/mg was significantly lower than that of normal group (54.27±9.53) μmol/mg (P<0.01).The significant negative correlation existed between the expression of NOSTRIN and the activity of eNOS in umbilical vessels of women with PE (r=-0.58,P<0.01).It was concluded that the level of NOSTRIN expression was increased in umbilical vessel of women with PE,indicating that it may be involved in the pathogenesis of PE.

作 者：
Wenpei XIANG Hanping CHEN Lian HU Xiaoyan XU  
作者单位：
Wenpei XIANG,Lian HU(Center of Reproductive Medicine,Institute of Family Planning Research,Tongji Hospital,Tong]i Medical College,Huazhong University of Science and Technology University,Wuhan 430030,China)
Hanping CHEN,Xiaoyan XU(Department of Obstetrics and Gynecology,Tongji Hospital,Tong]i Medical College,Huazhong University of Science and Technology University,Wuhan 430030,China) 
刊 名：
华中科技大学学报（医学）（英德文版）  SCI
英文刊名：
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY(MEDICAL SCIENCE) 
年，卷(期)：
2009 29(2) 
分类号：
R3 
关键词：
OSTRIN 知识脉络  pre-eclampsia 知识脉络  umbilical vessel 知识脉络  eNOS 知识脉络 
机标分类号：
O15 O4 
机标关键词：
normal groupnitric oxide synthasenegative correlationwomenlevelpossible roleeNOSsignificantproductsmRNAend 
基金项目：
 
DOI：
10.1007/s11596-009-0222-1 
参考文献(10条)
  1. Zimmermann K.Opitz N.Dedio J NOSTRIN:a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase 2002(26)
  2. LE Jie Gynecology 2004
  3. Shaamash AH.Elsnosy ED.Makhlouf AM Maternal and fetal serum nitric oxide (NO) concentrations in normal pregnancy,pre-eclampsia and eclampsia 2000(3)
  4. Seligman SP.Buyon JP.Clancy RM The role of nitric oxide in the pathogenesis of preeclampsia 1994(4)
  5. Var A.Yildirim Y.Onur E Endothelial dysfunction in preeclampsia.Increased homocysteine and decreased nitric oxide levels 2003(4)
  6. Roberts JM Endothelial dysfunction in preeclampsia 1998(1)
  7. Xiang WP.Chen HP.Xu XY Clone and Expression of NOSTRIN Gene in Human Umbilical Vein Endothelial Cell 2004(5)
  8. 相文佩.陈汉平 内皮型一氧化氮合酶运输诱导物NOSTRIN基因的克隆与表达 [期刊论文] -中国优生与遗传杂志2005(1)
  9. Xiang W P.Chert HP.Xu XY Expression of endothelial nitric oxide synthase traffic inducer in the placentas of women with preeclampsia 2005(2)
  10. Xiang WP.Chen HP.Guo YZ Study on the expression of endothelial nitric oxide synthase traffic inducer in the placenta of pregnancy induced hypertension syndrome 2006(2)

更多...

相似文献
相关博文