Characterization of pancreatic stem cells derived from adult human pancreas ducts by fluorescence activated cell sorting

doi：

Abstract：

AIM: To isolate putative pancreatic stem cells (PSCs)from human adult tissues of pancreas duct using serumfree, conditioned medium. The characterization of surface phenotype of these PSCs was analyzed by flow cytometry. The potential for pancreatic lineage and the capability of β-cell differentiation in these PSCs were evaluated as well.METHODS: By using serum-free medium supplemented with essential growth factors, we attempted to isolate the putative PSCs which has been reported to express nestin and pdx-1. The MatrigelTM was employed to evaluate the differential capacity of isolated cells. Dithizone staining, insulin content/secretion measurement, and immunohistochemistry staining were used to monitor the differentiation. Fluorescence activated cell sorting (FACS)was used to detect the phenotypic markers of putative PSCs.RESULTS: A monolayer of spindle-like cells was cultivated. The putative PSCs expressed pdx-1 and nestin.They were also able to differentiate into insulin-, glucagon-, and somatostatin-positive cells. The spectrum of phenotypic markers in PSCs was investigated; a similarity was revealed when using human bone marrow-derived stem cells as the comparative experiment, such as CD29,CD44, CD49, CD50, CD51, CD62E, PDGFR-α, CD73 (SH2),CD81, CD105(SH3).CONCLUSION: In this study, we successfully isolated PSCs from adult human pancreatic duct by using serumfree medium. These PSCs not only expressed nestin and pdx-1 but also exhibited markers attributable to mesenchymal stem cells. Although work is needed to elucidate the role of these cells, the application of these PSCs might be therapeutic strategies for diabetes mellitus.

作者：
Author：	Han-Tso Lin[1]  Shih-Hwa Chiou[2]  Chung-Lan Kao[3]  Yi-Ming Shyr[4]  Chien-Jen Hsu[5]  Yih-Wen Tarng[5]  Larry L-T Ho[6]  Ching-Fai Kwok[6]  Hung-Hai Ku[7]
作者單位：	Department of Medical Research and Education,Taipei Veterans General Hospital, Taipei, 11217, Taiwan, China Department of Medical Research and Education, Taipei Veterans General Hospital, and Institute of Clinical Medicine, National Yang-Ming University, Taipei, 11221, Taiwan,China Department of Physical Medicine and Rehabilitation, Taipei Veterans General Hospital, Taipei, 11217, Taiwan,China Department of Surgery, Taipei Veterans General Hospital, Taipei, 11217, Taiwan, China Department of Orthopedics,Kaohsiung Veterans General Hospital; Kaohsiung, 81346, Taiwan,China Division of Endocrinology and Metabolism, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, 11217, Taiwan, China Institute of Anatomy and Cell Biology, National Yang-Ming University, Taipei, Department of Education and Research, Taipei City Hospital, 11221, Taiwan, China
期 刊：	世界胃腸病學雜志（英文版）   ISTICSCI
Journal：	WORLD JOURNAL OF GASTROENTEROLOGY
年，卷(期)：	2006, 12(28)
分類號：	R5
Keywords：	Putative pancreas stem cell    Nestirr,pdx-1    Phenotypic marker
機標分類號：	R5 TB3
機標關鍵詞：	mesenchymal stem cells    diabetes mellitus    isolated cells    growth factors    flow cytometry
基金項目：	grants from the National Science Council，Yen-Tjing-Ling Medical Foundation，Foundation of Taipei Veterans General Hospital, Taiwan