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胰岛素分泌细胞的干细胞来源及其移植后命运

Insulin producing cells derived from stem cells and their fates after transplantation

目的:干细胞诱导分化成的胰岛素分泌细胞移植是治疗糖尿病的方法之一,但因其诱导方法尚未完善及植入体内后的存活分化命运不清等问题而未应用于临床.文章就胰岛素分泌细胞的干细胞来源及其植入体内的命运加以综述.资料来源:应用计算机检索PUBMED 1998-01/2007-03期间的相关文章,检索词为"insulin producing cell,cell transplantation,survival",并限定文章语言种类为English.同时计算机检索中国期刊全文数据库1996-01/2006-12期间的相关文章,检索词为"胰岛素分泌细胞,胚胎干细胞,成体干细胞",并限定文章语言种类为中文.资料选择:对资料进行初审,并查看每篇文献后的引文.纳入标准:文章所述内容应与胰岛素分泌细胞的干细胞来源或其植入体内后的存活分化相关.排除标准:重复研究.资料提炼:共收集到264篇相关文献,30篇文献符合纳入标准,排除的234篇文献为内容陈旧或重复.符合纳入标准的30篇文献中,分别涉及诱导为胰岛素分泌细胞的干细胞来源、胰岛素分泌细胞移植后的有效性、安全性、解决方法等内容.资料综合:糖尿病是由于胰岛素分泌不足及胰岛素抵抗造成糖代谢紊乱影响人类健康的主要疾病.不管是采用胰腺移植还是胰岛移植都因各种原因而限制了其在临床的应用,因此焦点放在了胰岛素分泌细胞上,包括其来源及移植后的命运.胰岛素分泌细胞来源于胚胎干细胞和成体干细胞,干细胞是一种具有自我更新,能够持续分裂而保持未分化状态,从而诱导分化为各种类型的细胞,因此有"种子细胞"之称.随着研究的深入,发现胰岛素分泌细胞的来源并不是问题,而问题的关键在于如何提高分化率及其移植后的命运,包括有效性、安全性以及解决方法.诱导产生的胰岛素分泌细胞表达活性低下、移植后其再生增殖能力差及容易被诱导凋亡等问题成为有效性最大的威胁,移植后的细胞易受免疫攻击和致瘤等问题也不容忽视.目前已开始着手以基因手段,从信号转导水平对其进行研究解决.结论:由胚胎干细胞和成体干细胞诱导分化的胰岛素分泌细胞,植入体内后有可能通过导入基因GLP-1,用表达B7-H4蛋白的质粒转染293T细胞和激活胰岛素/胰岛素样生长因子1等信号转导途径来解决有效性问题,但仍存在着安全性隐患,无法排除免疫抑制和致瘤的可能性.

作 者:
郭亮 唐军 屈纪富 徐海伟 Guo L Tang J Qu JF Xu HW  
作者单位:
郭亮,唐军,徐海伟,Guo L,Tang J,Xu HW(解放军第三军医大学生理学教研室,重庆市,400038)
屈纪富,Qu JF(解放军第三军医大学西南医院急诊科,重庆市,400038) 
刊 名:
中国组织工程研究与临床康复  ISTIC PKU
英文刊名:
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH 
年,卷(期):
2007 11(28) 
分类号:
R394.2 
关键词:
胚胎干细胞 知识脉络  成体干细胞 知识脉络  胰岛素分泌细胞 知识脉络  细胞移植 知识脉络  综述文献 知识脉络 
机标分类号:
R58 R73 
机标关键词:
胰岛素分泌分泌细胞细胞来源细胞移植命运胚胎干细胞成体干细胞细胞诱导分化资料文献安全性植入胰岛素样生长因子信号转导途径文章语言体内检索词方法标准期刊全文数据库 
基金项目:
国家自然科学基金 
DOI:
 
参考文献(31条)
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