首頁 > 期刊首頁 > 中國組織工程研究與臨床康復 > 2007年29期 > 構建糖尿病性肢端壞疽大鼠模型及中藥復方的干預效應
構建糖尿病性肢端壞疽大鼠模型及中藥復方的干預效應
Establishing a rat model of diabetic acromelic gangrene and intervention of Chinese compound
- doi：
- 摘要：
- 背景:糖尿病性肢端壞疽已成為糖尿病致殘、致死的重要原因之一.目的:建立糖尿病性肢端壞疽的大鼠模型,觀察中藥復方對糖尿病性肢端壞疽的干預作用.設計:對比觀察實驗.單位:河南科技大學臨床醫學院第一附屬醫院.材料:選用雄性Wistar大鼠50只,6周齡,體質量(200.0±16.3)g.以普通飼料單籠喂養,室溫18～22℃,自由攝食、飲水.隨機抽取10只作為空白對照組,其余40只用于造模.方法:實驗于2001-10/2002-04在河南科技大學動物房完成.①實驗分組:將40只大鼠禁食6 h后左下腹腹腔注射鏈脲佐菌素55.0 mg/kg;空白對照組注入同體積的檸檬酸鈉緩沖溶液.選用造模成功的20只大鼠,隨機分為2組:模型組和治療組,每組10只.治療組大鼠于造模成功后每天9:00灌胃中藥(60 g/kg),共3周;模型組灌胃等體積的生理鹽水.第3周末,各組大鼠禁食12 h,麻醉后處死取血測血糖、血脂和胰島素水平;實驗過程中記錄各組大鼠的每日飲水量.②實驗評估:肢端壞疽積分標準:以皮膚顏色發黑、皮膚有輕度開放性病灶、病灶已侵入深部肌肉組織3個等級對糖尿病性肢端壞疽大鼠的四肢進行評分,計算每只大鼠的總積分.對胰島β細胞分泌的功能進行評價主要觀察指標:實驗前后大鼠飲水量、體質量、三酰甘油、膽固醇、空腹血糖、血脂和胰島素水平的變化.結果:納入Wistar大鼠50只,造模不成功脫落20只,30只進入結果分析.①實驗期間,模型組和治療組飲水量明顯高于空白對照組(P＜0.01);治療組隨著治療時間增加飲水量明顯下降,模型組隨著時間的推移逐漸升高.②治療后模型組大鼠體質量明顯低于治療前(P＜0.01),治療組有下降趨勢,與治療前比較,差異不明顯(P＞0.05).③兩組大鼠均有明顯肢端壞疽出現(P＜0.01).治療組大鼠的體質量明顯高于模型組(P＜0.01),肢端壞疽情況明顯好于模型組(P＜0.01).④治療前治療組和模型組的空腹血糖水平明顯高于空白對照組(P＜0.01),而胰島素水平和胰島β細胞功能指數明顯低于空白對照組(P＜0.01).治療后兩組的空腹血糖水平高于空白對照組(P＜0.01),治療組的明顯低于模型組(P＜0.01),已接近正常值;兩組血脂和胰島β細胞功能指數均明顯低于空白對照組(P＜0.01),治療組明顯高于模型組(P＜0.01),接近正常值.⑤治療前其它兩組三酰甘油、膽固醇水平明顯高于空白對照組(P＜0.01).經過治療,治療組三酰甘油、膽固醇明顯下降(P＜0.01),與空白對照組比較,差異不明顯(P＞0.05);模型組繼續升高,明顯高于空白對照組(P＜0.01).結論:血清胰島素水平升高,血糖、血脂水平下降,可在一定程度上防治糖尿病足的發生、發展.該糖尿病性肢端壞疽模型對藥物反應敏感,可用于研究糖尿病足發病機制及藥物治療效果的評價.
- Abstract：
- BACKGROUND:Diabetic acromelic gangrene(diabetic foot)has become one of the important causes for the disability and death in diabetes mellitus.OBJECTIVE:To establish model of diabetic foot in rat,and observe the interventional effect of Chinese compound on diabetic foot.DESIGN:A comparative observational experiment.SETTING:The First Affiliated Hospital of Clinical Medical College,Henan University of Science and Technology.MATERIALS:Fifty male Wistar rats of 8 weeks old,(200.0±16.3)g,were raised with common feed in separate cage at the room temperature of 18-22℃.and they were free to access of feed and water.Ten rats were randomly selected as the blank control group,and the other 40 were used for model establishment.METHODS:The experiments were carried out in the Animal Room of Henan University of Science and Technology from October 2001 to April 2002.①Grouping:The 40 rats were fasted for 6 hours,and then treated with intraperitoneal injection of streptozotocin(55.0 mg/kg),while the 10 rats in the blank control group were injected with isovolume sodium citrate buffer solution.20 models were successfully established,and they were randomly divided into model group(n=10)and treatment group(n=10).Rats in the treatment group were treated for 3 weeks with intragastric perfusion of Chinese compound(60 g/kg)at 9:00 every day after model establishment,and those in the model group were given intragastric perfusion of isovolume saline.At the end of the third week,the rats were all killed under anesthesia after fasted for 12 hours,blood samples were collected to determine the levels of fasting blood glucose,blood lipids and insulin.The daily amount of drinking water was recorded in each group during the experiment.②Scoring standards for acromelic gangrenes:The limbs rats with diabetic foot were scored by three grades,including dark skin,mild open focus on skin,and focus had invaded deep muscular tissue.The total score of each rat was calculated.The beta-cell function index (HBCI)was also evaluated.MAIN OUTCOME MEASURES:The changes of the amount of drinking water,body mass and levels of triglyceride,cholesterol,fasting blood glucose,blood lipids and insulin were observed before and after treatment.RESULTS:Totally 50 Wistar rats were used.20 of them were excluded due to unsuccessful model establishment,and the other 30 rats were involved in the final analysis of results.①The amount of drinking water was obviously higher in the model group and treatment group than in the blank control group during the experiment(P＜0.01).As the treatmentlasted,the amount of drinking water was obviously decreased in the treatment group,but gradually increased in the model group.②After treatment,the body mass was obviously lower than that before treatment in the model group(P＜0.01).but had a descending trend without obvious difference as compared with that before treatment in the treatment group(P＞0.05).③Obvious acromelic gangrenes were obvious in both groups(P＜0.01).The body mass in the treatment group was obviously higher than that in the model group(P＜0.01),and the conditions of acromelic gangrene were obviously better than those in the model group(P＜0.01).④Before treatment,the levels of fasting blood glucose in the treatment group and model group were obviously higher than that in the blank control group(P＜0.01),while the levels of insulin and HBCl were obviously lower than those in the blank control group(P＜0.01).After treatment,the levels of fasting blood glucose in the treatment group and model group were obviously higher than that in the blank control group(P＜0.01),and it was obviously lower in the treatment group than in the model group(P＜0.01),it was close to the normal value in the treatment group.⑤The levels of triglyceride and cholesterol before treatment were obviously higher in the treatment group and model group than in the blank control group(P＜0.01).After treatment,the levels of triglyceride and cholesterol in the treatment group were obviously decreased(P＜0.01), which were not obviously different from those in the blank control group (P＞0.05), while those in the model group were increased continuously,and obviously higher than those in the blank control group(P＜0.01).CONCLUSION:Increasing the serum level of insulin and decreasing the levels of blood glucose and blood lipids can prevent and treat the occurrence and development of diabetic foot to some extent.This model of diabetic foot is sensitive to drug,and can be used to investigate the pathogenesis of diabetic foot and evaluate the effect of drug therapy.
參考文獻和引證文獻