首頁 > 期刊首頁 > 中國地方病學雜志 > 2008年3期 > 酪蛋白和高碘對小鼠甲狀腺形態結構的影響
酪蛋白和高碘對小鼠甲狀腺形態結構的影響
Effects of excessive iodine and casein on histomorphalogy and ultrastructure of mouse thyroid
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- 摘要：
- 目的 觀察酪蛋白和高碘對小鼠甲狀腺形態結構的影響.方法 按2×3析因設計將實驗小鼠分成6組,飲水分為適碘(50μg/L)、高碘(600μg/L)2個水平;飼料分為3個水平,Ⅰ:普通飼料,Ⅱ、Ⅲ:分別加入酪蛋白10%、20%的普通飼料.喂養12個月后,測定小鼠甲狀腺質量,光、電鏡觀察甲狀腺形態結構.結果 析因分析結果顯示碘因素明顯影響小鼠甲狀腺絕對質量和相對質量(F值分別為1623、9.47,P＜0.01),且酪蛋白與碘之間具有交互作用(F值分別為5.29、4.68,P＜0.01或＜0.05).600 Ⅰ組[(7.60±2.40)rag、(143.3-1-43.2)mg/kg]、600Ⅲ組[(8.63±1.88)mg、(166.2±39.4)mg/kg]分別與50Ⅰ組[(5.91±0.82)mg、(117.0±22.2)mg/kg]和50Ⅲ組[(4.90-t-0.63)mg、(106.1±13.3)ng/kg]比較,甲狀腺絕對質量和相對質量明顯增高(P＜0.05或＜0.01)或具有增高趨勢,而600Ⅱ組[(5.76±1.13)mg、(109.8±16.5)mg/kg]與600Ⅰ、600Ⅲ組比較,上述指標明顯減少(P＜0.05或＜0.01).光、電鏡下高碘組小鼠呈現高碘甲狀腺腫、淋巴細胞浸潤以及局部細胞代償性功能增強現象,而隨著酪蛋白攝入劑量增高,甲狀腺形態結構改變程度明顯減弱.結論 長期高碘可引發小鼠甲狀腺膠質潴留性腫大和炎性損傷,對甲狀腺炎的發生可能具有促進作用,而適當增加酪蛋白攝入可部分拮抗高碘引起的上述改變,對濾泡上皮細胞具有一定的保護作用.
- Abstract：
- Objective To observe the effects of casein and excessive iodine on histomorphalogY and ultrastructure of mouse thymicL Methods Based on 2 × 3 factorial design,the experimental mice were divided into 6 groupg Animal models were estabhshed by feeding the mice with different levels of iodine water and casein food.The levels of iodine were 50,600 μg/L in drinking water and 0(Ⅰ),10%(Ⅱ),20%(Ⅲ)of casein in food respectively.After 12 months,the thyroid weight was measured and the morphology of thyroid was observed under optical and electron microscope.Results Factorial analysis showed that iodine factors obviously affected the thyroid absolute and relative weiights of mice(F=16.23,9.47,P＜0.01),and there was interaction between casein and iodine(F=5.29,4.68,P＜0.01 or＜0.05).Compared wiht 150Ⅰ[(5.91±0.82)rag,(117.0±22.2)mg/kg]and 50Ⅲ[(4.90±0.63)rag,(106.1±13.3)mg/kg]groups.thyroid absolute and relative weights of the mice increased in 600 Ⅰ[(7.60±2.40)mg,(143.3±43.2)mg/kg]and 600Ⅲ[(8.63±1.88)mg,(166.2±39.4)mg/kg]groups(P＜0.05 or＜0.01),respectively.But compared with 600 Ⅰ and 600Ⅲ groups.they were reduced obviously in 600Ⅱ[(5.76±1.13)mg,(109.8±16.5)mg/kg]group(P＜0.05 or＜O.01).Colloid goiter,lymphocyte infiltration were found,some of the follicles epithelial cells appeared active under light and electron microscope in iodine excels group,which,however,decreased obviously along with the increase of casein dose.Conclusions Long-term excessive iodine may cause colloid goiter and inflammation injury of mice,possibly leading the development of thyroiditis in mice,which may be partly reduced by casein.
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