首页 > 学术期刊 > 中国生物化学与分子生物学报 > 2007年9期 > L-天冬酰胺酶表面呈现对P277肽免疫原性及抗NOD小鼠糖尿病作用的影响
L-天冬酰胺酶表面呈现对P277肽免疫原性及抗NOD小鼠糖尿病作用的影响
Effect of Displaying P277 Peptide on Surface of L-asparaginase on Its Antigenicity and Efficacy of Autoimmune Diabetes Prevention in NOD Mice
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将P277多肽融合在L-天冬酰胺酶的C端,中间引入破伤毒素肽(TTP),重组嵌合酶AnsB-TTP-P277能以可溶性蛋白的形式表达于大肠杆菌周质内,且能保持大约80%的天然酶催化活力.用纯化的嵌合酶腹腔注射非肥胖性(NOD)小鼠,可成功诱导小鼠体内产生高水平抗P277抗体.研究表明,P277肽可呈现于L-天冬酰胺酶表面,有效地克服单独P277的弱免疫原性,激发机体产生较强烈的免疫反应.小鼠胰腺病理学切片也显示:重组嵌合酶AnsB-TTP-P277和单纯P277肽相比,能更有效地抑制NOD小鼠糖尿病的发生.
- 作 者:
-
朱爱华
刘文涛
龙军
吴洁
刘景晶
李泰明
ZHU Ai-Hua
LIU Wen-Tao
Long Jun
Wu Jie
LIU Jing-Jing
LI Tai-Ming
- 作者单位:
- 朱爱华,ZHU Ai-Hua(中国药科大学生命科学与技术学院微基因药物实验室,南京,210009;徐州师范大学生命科学学院,江苏,徐州,221116)
刘文涛,龙军,吴洁,刘景晶,李泰明,LIU Wen-Tao,Long Jun,Wu Jie,LIU Jing-Jing,LI Tai-Ming(中国药科大学生命科学与技术学院微基因药物实验室,南京,210009)
- 刊 名:
- 中国生物化学与分子生物学报
ISTIC
PKU
- 英文刊名:
- CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY
- 年,卷(期):
- 2007 23(9)
- 分类号:
- Q789 R392
- 关键词:
- L-天冬酰胺酶
P277肽
胰岛素依赖型糖尿病
抗体
血糖
L-asparaginase
P277 peptide
insulin-dependent diabetes mellitus
antibody
blood glucose
- 机标分类号:
- R73 R57
- 机标关键词:
- 天冬酰胺酶表面呈现免疫原性小鼠胰腺糖尿病Escherichia colisoluble proteinimmune responseblood glucoseWestern blot酶催化活力可溶性蛋白重组形式表达小鼠体内免疫反应腹腔注射大肠杆菌非肥胖病理学
- 基金项目:
- 国家高技术研究发展计划(863计划),国家自然科学基金
- DOI:
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