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L-天冬酰胺酶表面呈现对P277肽免疫原性及抗NOD小鼠糖尿病作用的影响

Effect of Displaying P277 Peptide on Surface of L-asparaginase on Its Antigenicity and Efficacy of Autoimmune Diabetes Prevention in NOD Mice

将P277多肽融合在L-天冬酰胺酶的C端,中间引入破伤毒素肽(TTP),重组嵌合酶AnsB-TTP-P277能以可溶性蛋白的形式表达于大肠杆菌周质内,且能保持大约80%的天然酶催化活力.用纯化的嵌合酶腹腔注射非肥胖性(NOD)小鼠,可成功诱导小鼠体内产生高水平抗P277抗体.研究表明,P277肽可呈现于L-天冬酰胺酶表面,有效地克服单独P277的弱免疫原性,激发机体产生较强烈的免疫反应.小鼠胰腺病理学切片也显示:重组嵌合酶AnsB-TTP-P277和单纯P277肽相比,能更有效地抑制NOD小鼠糖尿病的发生.

作 者:
朱爱华 刘文涛 龙军 吴洁 刘景晶 李泰明 ZHU Ai-Hua LIU Wen-Tao Long Jun Wu Jie LIU Jing-Jing LI Tai-Ming  
作者单位:
朱爱华,ZHU Ai-Hua(中国药科大学生命科学与技术学院微基因药物实验室,南京,210009;徐州师范大学生命科学学院,江苏,徐州,221116)
刘文涛,龙军,吴洁,刘景晶,李泰明,LIU Wen-Tao,Long Jun,Wu Jie,LIU Jing-Jing,LI Tai-Ming(中国药科大学生命科学与技术学院微基因药物实验室,南京,210009) 
刊 名:
中国生物化学与分子生物学报  ISTIC PKU
英文刊名:
CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY 
年,卷(期):
2007 23(9) 
分类号:
Q789 R392 
关键词:
L-天冬酰胺酶 知识脉络  P277肽 知识脉络  胰岛素依赖型糖尿病 知识脉络  抗体 知识脉络  血糖 知识脉络  L-asparaginase 知识脉络  P277 peptide 知识脉络  insulin-dependent diabetes mellitus 知识脉络  antibody 知识脉络  blood glucose 知识脉络 
机标分类号:
R73 R57 
机标关键词:
天冬酰胺酶表面呈现免疫原性小鼠胰腺糖尿病Escherichia colisoluble proteinimmune responseblood glucoseWestern blot酶催化活力可溶性蛋白重组形式表达小鼠体内免疫反应腹腔注射大肠杆菌非肥胖病理学 
基金项目:
国家高技术研究发展计划(863计划),国家自然科学基金 
DOI:
 
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