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2型糖尿病 elisa tunel 主动脉 单克隆抗体 培养基 培养的 多药耐药 平滑 平滑肌细胞 激活 生长因子 组织培养 细胞学 罗格列酮 胰岛素抵抗 胸主动脉 血管平滑肌 转化生长因子 逆转

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首页 > 学术期刊 > 中国现代医学杂志 > 2007年6期 > 罗格列酮对大鼠血管平滑肌细胞凋亡和转化生长因子分泌的影响

罗格列酮对大鼠血管平滑肌细胞凋亡和转化生长因子分泌的影响

Effect of Rosiglitazone on secretion of transforming growth factor-β1 and apoptosis of vascular smooth musle cell of rat in vitro culture

目的 观察罗格列酮对大鼠胸主动脉平滑肌细胞(SMC)凋亡和转化生长因子-β1(TGF-β1)水平的影响.方法 采用罗格列酮处理体外培养的大鼠胸主动脉平滑肌细胞(SMC),用ELISA法检测培养基中转化生长因子-β1(TGF-β1)的水平,同时用流式细胞学和TUNEL法检测细胞凋亡.结果 罗格列酮诱导SMC快速分泌TGF-β1,在0.5 h内达到高峰,并且这种快速分泌可被GW9662逆转;罗格列酮诱导SMC凋亡存在浓度依赖,随着罗格列酮浓度的增加SMC凋亡率越来越高,100 μmol/L时SMC凋亡率达到最高,而超过100 μmol/L时,凋亡率并没有随着罗格列酮浓度的增加而增加,甚至有所下降,并且GW9662和anti-TGF-β1抗体均可逆转罗格列酮对SMC凋亡的诱导.结论 罗格列酮通过诱导的激活及转化生长因子β1(PPAR-γ)的快快速分泌促进血管平滑肌细胞凋亡.

作 者：
刘厂辉 游三丽 阳辉 郭慧 彭芳 LIU Chang-hui YOU San-li YANG Hui GUO Hui PENG Fang  
作者单位：
南华大学附一院,心内科,湖南,衡阳,421001 
刊 名：
中国现代医学杂志  ISTIC PKU
英文刊名：
CHINA JOURNAL OF MODERN MEDICINE 
年，卷(期)：
2007 17(6) 
分类号：
R332 
关键词：
TZD 知识脉络  TGF-β1 知识脉络  凋亡 知识脉络 
机标分类号：
R54 R73 
机标关键词：
罗格列酮大鼠血管平滑肌细胞凋亡转化生长因子分泌主动脉平滑肌细胞凋亡率浓度流式细胞学体外培养检测培养基抗体方法处理 
基金项目：
 
DOI：
 
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